Tech CEO Andy Byron has resigned after being caught in an embrace with his company's chief people officer at a Coldplay concert.
"Andy Byron has tendered his resignation, and the Board of Directors has accepted," Astronomer said in a statement.
"Our leaders are expected to set the standard in both conduct and accountability, and recently, that standard was not met."
Astronomer says it will begin searching for its next chief executive as the company's co-founder and chief product officer, Pete DeJoy, serves as interim CEO.
Mr Byron became embroiled in an internet scandal when a video of him embracing his chief people officer, Kristen Cabot, at a Massachusetts Coldplay concert went viral.
The pair were being filmed by concert crew, with the footage being projected on large screens.
Both Mr Byron and Ms Cabot, who had their arms around each other, became embarrassed, with Ms Cabot covering her face and Mr Byron dropping to the floor.
Coldplay singer Chris Martin can be heard saying, "either they're having an affair or they're just very shy".
As the video gained popularity, internet sleuths tried to identify the pair.
Astronomer then announced it would be investigating the matter and that any statements from Mr Byron circulating online were fake.
The following day, Astronomer announced Mr Byron had been placed on leave.
The ABC has contacted Astronomer to ask if Ms Cabot would also be placed on leave.
In its announcement of Mr Byron's resignation, the New York-based tech company acknowledged the viral nature of events.
"Before this week, we were known as a pioneer in the DataOps space," Astronomer said.
"While awareness of our company may have changed overnight, our product and our work for our customers have not."
Mr Bryon has since been removed from the leadership team on Astronomer's website but remains visible on the company's board of directors.
Catherine Lumby, a media professor at Sydney University, says the scale of coverage the story garnered would have weighed on Astronomer to act quickly.
"It's very hard to get that toothpaste back in the tube once it's out," she told ABC News Channel.
"This is well beyond just brand management and reputation management in its traditional sense.
"Social media can pick something up and it's like a tsunami once it takes hold."
Online reaction
It took just one 15-second video posted to TikTok for the moment to ignite the internet.
Concertgoer Grace Springer was filming the Coldplay gig the moment the camera locked on Mr Byron and Ms Cabot and captured their now-infamous reaction.
She then uploaded the clip to her TikTok account under her username @instaagrace.
As of Sunday morning, the video has been viewed more than 100.4 million times and has garnered 8.9 million likes.
The internet was quick to respond, with countless reactions flooding social media.
"I don't know what's worse. Getting caught cheating or getting caught at a Coldplay concert," one user wrote on X.
Another wrote: "This story is absolutely unremarkable except in how it managed to combine almost everything it's socially acceptable to hate brilliantly: HR, Coldplay, cheaters, CEOs, millionaires".
Ms Springer spared a thought for the partners of Mr Byron and Ms Cabot.
"A part of me feels bad for turning these people's lives upside down, but play stupid games … win stupid prizes," Ms Springer told UK tabloid The Sun.
"I hope their partners can heal from this and get a second chance at the happiness they deserve with their future still in front of them.
"I hope, for them, my video was a blessing in disguise."